The antibodies have been generated to the N-terminus of Aβ 42. Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by certain secretases. We will contact you within 24 hours. Sample Type: Cerebrospinal Fluid, 10 µL Supernatant, 10 µL Tissue Homogenate, 10 µL. Product name: Amyloid beta 42 Mouse ELISA Kit. The affinity purified monoclonal antibody absorbed on the plate and the affinity purified polyclonal-HRP detection antibody were generated to ABeta 1-42. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Sample 1:2 1:4 1:8 serum(n=5) 87-103% 85-99% 85-101% EDTA plasma(n=5) 82-89% 86-98% 83-101% heparin plasma(n=5) 82-93% 82-96% 83-96%. It is based upon a Sandwich assay principle and can be used to detect levels of APP / Beta Amyloid Precursor as low as 0. Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology. " Add sample to plate, incubate overnight. Isotype and Serum Controls. Amyloid beta 42 mouse elisa kit. Components 1 x 96 tests 1100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Human Amyloid beta detection antibody 2 vials Human Amyloid beta Standards (lyophilized) 2 vials Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml. The results were demonstrated by the percentage of calculated concentration to the expected.
Highly selective to mouse and rat amyloid-beta 1-42 with minimal cross reactivity to human amyloid-beta 1-42 (see data below). Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. Sandwich ELISA, HRP-labelled antibody. Product information. 2015) "Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors. " Product DescriptionThe oligomeric form of Amyloid Beta peptide (Aβ, 1-42) has been closely linked to Alzheimer's Disease. Detection Target: Mouse Amyloid Beta Precursor Protein Binding Protein B3. Loading... company name: Invitrogen. Storage Conditions: Store all components at 4°C. Mouse Amyloid Beta Precursor Protein Binding Protein B3 ELISA Kit –. Warning: Last items in stock! Apr 24;12:16 Application: Mouse lysate. Combes M, Poindron P, Callizot N. (2015) "Glutamate protects neuromuscular junctions from deleterious effects of β-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture. " From product page to cart, your experience is curated to be fast, efficient and safe. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to Aβ residues 1-4 and is highly specific just to amyloid beta peptide.
Epub ahead of print]. Áreas de investigación. Aβ40 ELISA Kit allows for the in vitro quantitative determination of Aβ40 concentrations in serum, plasma, tissue homogenates and other biological fluids. International delivery is also available: please inquire for costs. Amyloid beta elisa kit mouse change. Neurobiology of Disease. Standards and samples are pipetted into the wells and the immobilized antibody captures Amyloid beta 1-40 present in the samples.
Product Name: Description: Beta amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein by a protease, gamma-secretase. Your browser version seems rather old, and we don't support it. FroggaBio More About Us. Shipping: Overnight delivery in styrofoam box with ice packs. Epub ahead of print] Application: Human. Kit ComponentsThe ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols. FroggaBio - Homepage. Alternative NamesBeta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. LS-F8583 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse APP / Beta Amyloid Precursor in samples of Plasma, Serum and Tissue Homogenates. Questions and comments. Detection MethodColorimetric.
Customer Service (800) 786-5777. FroggaBio's trained staff are here to support your every need. Immunogen DescriptionThe standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. Front Synaptic Neurosci. Application: Synthetic constructs. 56-100 pg/ml assay range. Amyloid beta elisa kit mouse trap. The Biosensis o-Aβ Elisa detects Aβ oligomers as validated and described by Youmans KL et al (2012) in mouse and by Combes M et al (2015), in rat and human samples. At ambient temperature. ¿Olvidaste tu contraseña? AntigenAmyloid Beta Peptide 1-40 (Ab1-40). IHC/ICC Slides and Lysates for Westerns.
Upon receipt, store the product at the temperature recommended below. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-Aβ capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. Kasus-Jacobi A et al. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. Safety: Stop solution contains acid. 15(12): 2270-2 Application: Rat PC12 RIPA cell extract.
Batch NumberPlease see item label. Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice. " Availability: In stock. Application: Tris-extracts of EFAD transgenic mouse brains.
After appropriate washing steps, an anti-APBB3 detection antibody conjugated to biotin binds to the captured analyte. Sandwich ELISA, Double Antibody. Sample typeCell culture supernatant, Serum, Plasma. ELISA plate is coated with an affinity purified monoclonal antibody that recognizes mouse and rat amyloid-beta 1-42. Species ReactivityHuman, Rat. APBB3 present in samples reacts with an anti-Mouse APBB3 capture antibody coated and dried on a microtiter plate. Please include any relevant information (product name, etc). Add color reagents, 30 min. Whether you have a product question or require order assistance we are ready to help.
The kit detects amyloid-beta 1-42 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract. Acta Neuropathol Commun. Company information. Sample TypeCSF, Tissue Homogenates.
Background on Amyloid-Beta (1-42). Store the complete kit at 2–8°C. Reagents for up to 96 tests. Volume 37, January 2016, Pages 47-57 Application: Tris-extracts of E3FAD and E4FAD transgenic mouse brains. Note: This product is for research use only. Application(s)ELISA.
This product can not be ordered from your electronic catalog. 93(4):633-43 Application: Native Rat neurites & human muscle cell co-culture supernatants. Custom peptides & Antibodies. Equilibrate plate to RT. Chem Soc Rev 49:5446-5472 (2020).
Application DetailsELISA. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease. " PlatformPre-coated microplate (12 x 8 well strips). Flow cytometry antibodies.
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